Complete secretion of activable bovine prochymosin by genetically engineered L forms of Proteus mirabilis

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Complete secretion of activable bovine prochymosin by genetically engineered L forms of Proteus mirabilis.

To circumvent problems encountered in the synthesis of active chymosin in a number of bacteria and fungi, a recombinant DNA L-form expression system that directed the complete secretion of fully activable prochymosin into the extracellular culture medium was developed. The expression plasmid constructions involved the in-frame fusion of prochymosin cDNA minus codons 1 to 4 to streptococcal pyro...

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Reversion of L forms and spheroplasts of Proteus mirabilis.

Altenbern, Robert A. (U.S. Army Chemical Corps, Fort Detrick, Frederick, Md.). Reversion of L forms and spheroplasts of Proteus mirabilis. J. Bacteriol. 85:269-272. 1963.-Spheroplasts or 3-B type L forms of Proteus mirabilis formed by growth in the presence of penicillin are able to revert to the bacillary form, as judged by regain of resistance to osmotic shock. Reversion takes place in the ab...

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Reversion of 3A type L forms of Proteus mirabilis.

Reversion of L forms to the normal bacterial state has been described in general terms in a variety of bacterial cell-L-form systems (Dienes and Weinberger, 1951). The relatively large 3B type L colony formed on penicillin-containing medium is composed entirely of spherical bodies of varying sizes (2 to 10 ,u in diameter) that appear to propagate by a budding process. In contrast, 3A type L for...

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Critical factors influencing growth of L forms of Proteus mirabilis.

The observations, primarily by Dienes and associates, of L colonies arising spontaneously or by penicillin induction from species of Proteus or Salmonella led to the description of two morphologically distinct colonial forms, designated as the 3B type and the 3A type. It has been reported further that the viable elements of these two types exhibit markedly dissimilar properties such as size, nu...

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Synergism of polymyxin and sulfonamides in L-forms of Staphylococcus aureus and Proteus mirabilis.

Earlier workers suggested that synergism of polymyxin and sulfonamides occurred because sulfonamides produced changes in the cell wall which permitted the more ready access of polymyxin. We tested this hypothesis by using L-forms, which lack cell wall. The demonstration that synergism of polymyxin and sulfonamides occurs in L-forms of Proteus mirabilis and Staphylococcus aureus suggested that t...

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ژورنال

عنوان ژورنال: Applied and Environmental Microbiology

سال: 1989

ISSN: 0099-2240,1098-5336

DOI: 10.1128/aem.55.4.1009-1015.1989